BIMX are produced by WT phage infecting WT cells and BIMX 2 are produced by CEMX infecting BIMX. Solid black lines, phage adsorption and replication broken red lines - phage adsorption and loss. Panel A: Infection and phage replication relationships. WT, Wild type bacteria and phage BIMX and CEMX, bacteria with spacers for acquired resistance to WT phage and the first-order CRISPR Escape mutants (CEMX), respectively BIMX 2 and CEMX 2, bacteria with spacers for acquired resistance to CEMX and the second-order CRISPR–escape mutants, respectively. Ellipses - bacteria Pentagons - phage red and blue rectangles - acquired spacers red and blue circles, regions of the phage genome corresponding to the acquired spacers (protospacers) stars, mutated protospacers generating CEMs.
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